Apaf1 (CED-4 Homolog) Regulates Programmed Cell Death in Mammalian Development

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Apaf1 (CED-4 Homolog) Regulates Programmed Cell Death in Mammalian Development

The cytosolic protein APAF1, human homolog of C. elegans CED-4, participates in the CASPASE 9 (CASP9)-dependent activation of CASP3 in the general apoptotic pathway. We have generated by gene trap a null allele of the murine Apaf1. Homozygous mutants die at embryonic day 16.5. Their phenotype includes severe craniofacial malformations, brain overgrowth, persistence of the interdigital webs, and...

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Programmed cell death (PCD) in mammals has been implicated in several disease states including cancer, autoimmune disease, and neurodegenerative disease. In Caenorhabditis elegans, PCD is a normal component of development. We find that Salmonella typhimurium colonization of the C. elegans intestine leads to an increased level of cell death in the worm gonad. S. typhimurium-mediated germ-line ce...

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Translocation of C . elegans CED - 4 to Nuclear Membranes During Programmed Cell Death

G. L. MacLean, Cimbebasia 2, 163 (1974); D. Robinson, Emu 90, 40 (1990); and R. E. Major, Emu 91, 236 (1991). 5. A. F. Skutch, Ibis 91, 430 (1949). 6. T. H. Clutton-Brock, The Evolution of Parental Care (Princeton Univ. Press, Princeton, NJ, 1991). 7. I. Rowley and E. Russell, in Bird Population Studies: Relevance to Conservation and Management, C. M. Perrins, J.-D. Lebreton, G. J. M. Hirons, E...

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Translocation of C. elegans CED-4 to nuclear membranes during programmed cell death.

The Caenorhabditis elegans Bcl-2-like protein CED-9 prevents programmed cell death by antagonizing the Apaf-1-like cell-death activator CED-4. Endogenous CED-9 and CED-4 proteins localized to mitochondria in wild-type embryos, in which most cells survive. By contrast, in embryos in which cells had been induced to die, CED-4 assumed a perinuclear localization. CED-4 translocation induced by the ...

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ژورنال

عنوان ژورنال: Cell

سال: 1998

ISSN: 0092-8674

DOI: 10.1016/s0092-8674(00)81732-8